Diversity and sensitivity to antibiotics of different species of Pseudomonas at Hôpital de l'Amitié Sino-Guinéenne, Kipé/Conakry

Abstract

Introduction: Infections caused by different species of Pseudomonas are amongst leading causes of morbidity
and mortality worldwide. The aim of this study was to determine different species of Pseudomonas isolated from
biological fluids between Jun 2014 and Jun 2018 at China-Guinea friendship hospital of Kipé/Conakry, and to
describe their antibiotic sensitivity patterns. Material and methods: Bacterial identification and antibiograms as
well as determination of minimal inhibitory concentrations (MICs) were performed on Vitek2 Compact 15.
Results: Seventy-five isolates of Pseudomonas belonging to six different species were identified: Pseudomonas
aeruginosa (44), Pseudomonas luteola (15), Pseudomonas fluorescens (13), Pseudomonas mendocina (1),
Pseudomonas oryzihabitans (1) and Pseudomonas putida (1). The patients mean age was 49,12±23,48 years [18
days-90 years] and the sexe-ratio (M/F) = 0,875. The majority of the isolates was susceptible to imipenem
(93,33%), amikacin (88,00%), gentamicin (72,00%), tobramycin (70,66%), piperacillin/tazobactam (70,66%),
ceftazidime (60,00%), cefotaxime (54,66%), ofloxacin (57,33%) and ciprofloxacin (58,66%). On the other
hands, these isolates were resistant to ampicillin (89,33%), trimethoprim/sulfamethoxazol (89,33%), nalidixic
acid (85,33%), cefalotin (77,33%), ticarcillin (68,00%), cefoxitin (64,00%), and nitrofurantoine (57,33%).
Multidrug-resistance patterns with high MIC levels were observed particularly for the isolate N°64 from a
urinary tract infection of 82 years old patient. This isolate was resistant to all antibiotics tested. Conclusion: Our
study revealed 75 isolates of Pseudomonas belonging to six species, carrying a multidrug-resistance phenotype
to all antibiotic families tested in Guinea